By Walter Maechtle
This e-book is split into chapters overlaying instrumentation, sedimentation speed runs, density gradient runs, software examples and destiny advancements. particularly, the unique program bankruptcy demonstrates the flexibility and tool of AUC through many attention-grabbing and critical business examples. therefore the ebook concentrates on functional features instead of info of centrifugation concept.
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Extra resources for Analytical Ultracentrifugation of Polymers and Nanoparticles (Springer Laboratory)
16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. Svedberg T, Pedersen KO (1940) Die Ultrazentrifuge. edu Bowen TJ, Rowe AJ (1970) An introduction to ultracentrifugation. Wiley, London Lewis MS, Weiss GH (1976) Proc Conf Fifty Years of the Ultracentrifuge, 24–26 February 1975, Bethesda, ML, Biophys Chem 5:1–286 Mächtle W (1999) Biophys J 76:1080 Müller HG (1999) Bayer AG, Leverkusen, Germany, personal communication Mächtle W (1999) Prog Colloid Polym Sci 113:1 Börger L, Lechner MD, Stadler M (2004) Prog Colloid Polym Sci 127:19 Schachman HK (1959) Ultracentrifugation in biochemistry.
Rather, only the back-scattered ﬂuorescence light is measured by step-wise radial scanning of the whole surface of the cell along a sector. This is reached by a confocal setup of the detector geometry . 12 shows a measurement with Laue’s ﬂuorescence detector. Fig. 12. 4 Detectors 41 Turbidity Detectors Similarly to the case of the ﬂuorescence detector, to our knowledge there are just two types of turbidity (= light scattering) detectors [13, 14]. Both are user-made, and therefore not commercially available.
The design of the modern Beckman Optima XL-A/I series accommodates the continuous use of the existing cells of the preliminary Model E centrifuge (with exception of the 30-mm cell). Only small modiﬁcations have been added to XL-A/I cells, such as smaller window holders, different window material (quartz of higher purity), or anodized screw rings. Some of the most important parts of AUC cells in the case of metal centerpieces, such as aluminum or titanium, are ﬂat elastic gaskets between the centerpieces and the two glass windows (mostly cut out of thin polyethylene foil).
Analytical Ultracentrifugation of Polymers and Nanoparticles (Springer Laboratory) by Walter Maechtle